OTUD4 Antibody

ProSci
Product Code: PSI-42-069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-069-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
OTUD4, OTU domain containing 4, DKFZp434I0721, DUBA6, HIN1, HSHIN1, KIAA1046, HIV-1 induced protein HIN-1, OTU domain containing 4 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 90kDa band in Human Liver and Ovary lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 117kDa according to NP_955356.1and NP_001096123.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Wu JM, Fackler MJ, Halushka MK, Molavi DW, Taylor ME, Teo WW, Griffin C, Fetting J, Davidson NE, De Marzo AM, Hicks JL, Chitale D, Ladanyi M, Sukumar S, Argani P. Heterogeneity of breast cancer metastases: comparison of therapeutic target expression and promoter methylation between primary tumors and their multifocal metastases. Clin. Cancer Res. 2008 Apr 14 (7): 1938-46.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-SRSRDEGYQYHRNVR
NCBI Gene ID #:
54726
NCBI Official Name:
OTU deubiquitinase 4
NCBI Official Symbol:
OTUD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_955356.1, NP_001096123.1
Protein GI Number:
40807496
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize reported isoforms 1 (NP_955356.1) and 3 (NP_001096123.1).
Swissprot #:
Q01804