Oxt Antibody

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ProSci
Product Code: PSI-42-523
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-523-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-523 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear s
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42-523 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear s

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Further Information

Additional Names:
Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 22kDa band in Mouse Liver after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9kDa according to NP_035155.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: DGCRTDPACDPES
NCBI Gene ID #:
18429 (mouse); 25504 (rat);
NCBI Official Name:
oxytocin
NCBI Official Symbol:
Oxt
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_035155.1
Protein GI Number:
6754960
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
P35454