P2RX7 Antibody

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ProSci
Product Code: PSI-42-772
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-772-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-772 staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
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42-772 staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
P2RX7, purinergic receptor P2X, ligand-gated ion channel, 7, MGC20089, P2X7, ATP receptor, P2X purinoceptor 7, P2X7 receptor, P2Z receptor, purinergic receptor P2X7
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: YETNKVTRIQSMNY-C
NCBI Gene ID #:
5027
NCBI Official Name:
purinergic receptor P2X, ligand-gated ion channel, 7
NCBI Official Symbol:
P2RX7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002553.2
Protein GI Number:
29294631
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q99572