PALLD Antibody

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ProSci
Product Code: PSI-42-052
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-052-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-052 (0.1ug/ml) staining of Human Umbillical Cord lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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42-052 (0.1ug/ml) staining of Human Umbillical Cord lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PALLD, palladin, cytoskeletal associated protein, CGI-151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, PNCA1, SIH002, palladin, sarcoma antigen NY-SAR-77
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 125kDa band observed in Human Umbillical Cord lysates (calculated MW of122kDa according to NP_057165.3). Recommended concentration: 0.1-0.3ug/ml. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Background References:
  • Bare LA, Morrison AC, Rowland CM, Shiffman D, Luke MM, Iakoubova OA, Kane JP, Malloy MJ, Ellis SG, Pankow JS, Willerson JT, Devlin JJ, Boerwinkle E. Five common gene variants identify elevated genetic risk for coronary heart disease. Genet. Med. 2007 Oct 9 (10): 682-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RDSGDENEPIQER
NCBI Gene ID #:
23022
NCBI Official Name:
palladin, cytoskeletal associated protein
NCBI Official Symbol:
PALLD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057165.3
Protein GI Number:
93102361
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q8WX93