PAM Antibody

ProSci
Product Code: PSI-46-696
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-696-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-696 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-696 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PAM, PAL, peptidylglycine alpha-amidating monooxygenase , PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Kidney lysates (calculated MW of 101kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Glauder J, Ragg H, Rauch J, Engels JW. Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells. Biochem Biophys Res Commun. 1990 Jun 15;169(2):551-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DQEKEDDGSESEE
NCBI Gene ID #:
5066
NCBI Official Name:
peptidylglycine alpha-amidating monooxygenase
NCBI Official Symbol:
PAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000910.2, NP_620121.1, NP_620176.1, NP_620177.1
Protein GI Number:
21070984
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
Swissprot #:
P19021