PAR1 Antibody

ProSci
Product Code: PSI-46-994
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-994-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-994 (0.3ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-994 (0.3ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
F2R, coagulation factor II (thrombin) receptor, CF2R, HTR, PAR1, TR, coagulation factor II receptor, protease-activated receptor 1, thrombin receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Niessen F, Schaffner F, Furlan-Freguia C, Pawlinski R, Bhattacharjee G, Chun J, Derian CK, Andrade-Gordon P, Rosen H, Ruf W. Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature. 2008 Apr 3;452(7187):654-8. Epub 2008 Feb 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TRARRPESKATNAT
NCBI Gene ID #:
2149
NCBI Official Name:
coagulation factor II (thrombin) receptor
NCBI Official Symbol:
F2R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001983.2
Protein GI Number:
166362740
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Innate Immunity
SPECIFICITY:
.
Swissprot #:
P25116