Pard3b Antibody

ProSci
Product Code: PSI-42-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-922-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-922 (0.5ug/ml) staining of Mouse fetal Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-922 (0.5ug/ml) staining of Mouse fetal Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Pard3b, par-3 partitioning defective 3 homolog B (C. elegans), DN-276O13.2, 1810008K04Rik, 2010002N16Rik, 2810455B10Rik, Als2cr19, PAR3B, PAR3L, PAR3beta, RP23-20G7.1, DN-276O13.2, PAR3-L protein, PAR3-beta, amyotrophic lateral sclerosis 2 chromosomal region candidate gene 19 protein homolog, partitioning defective 3 homolog B, partitioning defective 3-like protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 125kDa band observed in Mouse fetal Kidney lysates (calculated MW of 133kDa according to NP_001074519.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-RNQDPRQKNP
NCBI Gene ID #:
72823 (mouse); 301455 (rat);
NCBI Official Name:
par-3 family cell polarity regulator beta
NCBI Official Symbol:
Pard3b
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 125 kDa
Protein Accession #:
NP_001074519.2
Protein GI Number:
163310767
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q9CSB4