Parvalbumin Antibody

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ProSci
Product Code: PSI-46-135
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-135-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-135 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer).r. Detected by chemiluminescence.
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46-135 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fo
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46-135 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer).r. Detected by chemiluminescence.
46-135 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fo

Documents

Further Information

Additional Names:
parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1). Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3ug/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Background References:
  • Hashimoto T, Bergen SE, Nguyen QL, Xu B, Monteggia LM, Pierri JN, Sun Z, Sampson AR, Lewis DA. Relationship of brain-derived neurotrophic factor and its receptor TrkB to altered inhibitory prefrontal circuitry in schizophrenia. J Neurosci. 2005 Jan 12;25(2):372-83.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-GVDEFSTLVAES
NCBI Gene ID #:
5816
NCBI Official Name:
parvalbumin
NCBI Official Symbol:
PVALB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002845.1
Protein GI Number:
4506335
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Reported variants represent identical protein: NP_002845.1, NP_001302461.1
Swissprot #:
P20472