PAX8 Antibody

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ProSci
Product Code: PSI-46-141
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-141-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-141 (1ug/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-141 (1ug/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PAX8, paired box gene 8, paired domain gene 8, paired box 8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney Cortex. Recommended concentration: 5ug/ml.
Background References:
  • Pasca di Magliano M, Di Lauro R, Zannini M. Pax8 has a key role in thyroid cell differentiation. Proc Natl Acad Sci USA. 2000 Nov 21;97(24):13144-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: PHNSIRSGHGGLN-C
NCBI Gene ID #:
7849
NCBI Official Name:
paired box 8
NCBI Official Symbol:
PAX8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1
Protein GI Number:
5803117
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Swissprot #:
Q06710