PDE5A Antibody
| Code | Size | Price |
|---|
| PSI-46-153-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Background References:
- Morelli A, Filippi S, Mancina R, Luconi M, Vignozzi L, Marini M, Orlando C, Vannelli GB, Aversa A, Natali A, Forti G, Giorgi M, Jannini EA, Ledda F, Maggi M. Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa. Endocrinology. 2004 May;145(5):2253-63. Epub 2004 Feb 05.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LINGESGQAKRN
NCBI Gene ID #:
8654
NCBI Official Name:
phosphodiesterase 5A, cGMP-specific
NCBI Official Symbol:
PDE5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223)
Protein GI Number:
61744435
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
Swissprot #:
O76074