PDK1 Antibody

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ProSci
Product Code: PSI-46-155
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-155-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-155 (1ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-155 (1ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PDK1, pyruvate dehydrogenase kinase, isoenzyme 1, HGNC:8809, mitochondrial pyruvate dehydrogenase kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1, OTTHUMP00000205076, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyruvate dehydrogenase kinase 1,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 65-70kDa band observed in Rat Heart lysates (calculated MW of 49.2kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Background References:
  • Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712):1098-101.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DFKDKSAEDAK
NCBI Gene ID #:
5163
NCBI Official Name:
pyruvate dehydrogenase kinase, isozyme 1
NCBI Official Symbol:
PDK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002601.1
Protein GI Number:
4505689
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q15118