PDPN Antibody

ProSci
Product Code: PSI-42-765
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-765-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-765 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-765 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PDPN, podoplanin, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, OTTHUMP00000009640, OTTHUMP00000044504, glycoprotein, 36-KD, hT1alpha-1, hT1alpha-2, lung type I cell membrane associated glycoprotein, lung type-I cell membrane-associated glycoprotein, lung type-I cell membrane-associated glycoprotein (T1A-2)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Experiments gave bands at approx 25kDa and 37kDa in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. The 37kDa band corresponds to earlier findings in literature with different antibodies (Boucherot et al, Nephrol Dial Transplant. 2002 Jun;17(6):978-84; PMID: 12032185). This protein has a calculated MW of 24.9kDa according to NP_006465.3. Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-EKVDGDTQTTVEKD
NCBI Gene ID #:
10630
NCBI Official Name:
podoplanin
NCBI Official Symbol:
PDPN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006465.3, NP_938203.2, NP_001006625.1, NP_001006626.1
Protein GI Number:
54792056
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).
Swissprot #:
Q86YL7