PFKFB2 Antibody
| Code | Size | Price |
|---|
| PSI-42-201-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PFKFB2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, OTTHUMP00000034285, OTTHUMP00000034286, PFKFB, cardiac, fructose-2,6-bisphosphatase, cardiac isozyme
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
Background References:
- Arden C, Hampson LJ, Huang GC, Shaw JA, Aldibbiat A, Holliman G, Manas D, Khan S, Lange AJ, Agius L, A role for PFK-2/FBPase-2, as distinct from fructose 2, 6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells. Biochem J. 2008 Apr 1;411(1):41-51.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: SEQNNNSYETKTP-C
NCBI Gene ID #:
5208
NCBI Official Name:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
NCBI Official Symbol:
PFKFB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006203.2, NP_001018063.1
Protein GI Number:
64762406
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
Swissprot #:
O60825