PLA2G16 Antibody

ProSci
Product Code: PSI-46-856
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-856-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-856 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-856 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
PLA2G16, phospholipase A2, group XVI, AdPLA, H-REV107-1, HRASLS3, HREV107, HREV107-3, MGC118754, HRAS-like suppressor 3, adipose-specific PLA2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0kDa according to NP_009000.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Background References:
  • Nazarenko I, Kristiansen G, Fonfara S, Guenther R, Gieseler C, Kemmner W, Schafer R, Petersen I, Sers C. H-REV107-1 stimulates growth in non-small cell lung carcinomas via the activation of mitogenic signaling. Am J Pathol. 2006 Oct;169(4):1427-39.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: RAPIPEPKPGDLIE-C
NCBI Gene ID #:
11145
NCBI Official Name:
phospholipase A2, group XVI
NCBI Official Symbol:
PLA2G16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009000.2
Protein GI Number:
189571618
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).
Swissprot #:
P53816