PLA2G4A Antibody

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ProSci
Product Code: PSI-42-609
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-609-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-609 (1ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-609 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-609 (1ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-609 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QEKTFRQQRKEHIR
NCBI Gene ID #:
5321
NCBI Official Name:
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol:
PLA2G4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_077734.1
Protein GI Number:
23943920
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Immunology
SPECIFICITY:
.
Swissprot #:
P47712