PLA2G4F Antibody

ProSci
Product Code: PSI-42-796
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-796-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
PLA2G4F, phospholipase A2, group IVF, DKFZp666G192, DKFZp781B229, PLA2G4FZ, cytosolic phospholipase A2 zeta, cPLA2-zeta
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QTAEEKAFGDFVINR
NCBI Gene ID #:
255189
NCBI Official Name:
phospholipase A2, group IVF
NCBI Official Symbol:
PLA2G4F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_998765.3
Protein GI Number:
281371376
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q68DD2