POU2F1 Antibody

ProSci
Product Code: PSI-42-575
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-575-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-575 (0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-575 (0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
POU2F1, POU class 2 homeobox 1, OCT1, OTF1, OTTHUMP00000032348, Octamer-binding transcription factor-1, POU domain, class 2, transcription factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-NVQSKSNEESGDSQQ
NCBI Gene ID #:
5451
NCBI Official Name:
POU class 2 homeobox 1
NCBI Official Symbol:
POU2F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002688.2
Protein GI Number:
42476164
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription
SPECIFICITY:
.
Swissprot #:
P14859