PPID Antibody

ProSci
Product Code: PSI-46-209
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-209-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-209 (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-209 (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CYP-40, MGC33096, peptidylprolyl isomerase D (cyclophilin D) , 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase, cyclophilin 40, cyclophilin D, cyclophilin-related protein, peptidylprolyl isomerase D, rotamase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.8kDa according to NP_005029.1). In transfected HEK293 transiently expressing CyP-40 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Forte M, Gold BG, Marracci G, Chaudhary P, Basso E, Johnsen D, Yu X, Fowlkes J, Bernardi P, Bourdette D. Cyclophilin D inactivation protects axons in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Proc Natl Acad Sci USA. 2007 May 1;104(18):7558-63. Epub 2007 Apr 26.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DENFHYKHDREG
NCBI Gene ID #:
5481
NCBI Official Name:
peptidylprolyl isomerase D
NCBI Official Symbol:
PPID
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005029.1
Protein GI Number:
4826932
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q08752