IL-4 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5190-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-4 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
Il4e12, Il-4, Interleukin-4, B-cell IGG differentiation factor, IL-4
Application Note:
ELISA:
To detect Rat IL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-4.
Sandwich:
To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-4 (XP-5190Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.
Western Blot:
To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-4.
Sandwich:
To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-4 (XP-5190Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.
Western Blot:
To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of na?ve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-4 (Rat Interleukin-4).
NCBI Gene ID #:
287287
NCBI Official Name:
interleukin 4
NCBI Official Symbol:
Il4
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P20096
Protein GI Number:
124339
Purification:
Anti-Rat IL-4 specific antibody was purified by affinity chromatography employing immobilized Rat IL-4 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P20096
User NOte:
Centrifuge vial prior to opening.