PTPRM Antibody

ProSci
Product Code: PSI-46-252
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-252-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-252 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-252 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
PTPRM, protein tyrosine phosphatase, receptor type, M, MGC166994, PTPRL1, R-PTP-MU, RPTPM, RPTPU, hR-PTPu, protein tyrosine phosphatase mu, protein tyrosine phosphatase, receptor type, mu polypeptide
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 110kDa and 16kDa in Rat Retina lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165kDa according to Human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Uterus and Thyroid. Recommended concentration: 3.75ug/ml.
Background References:
  • Aricescu AR, Hon WC, Siebold C, Lu W, van der Merwe PA, Jones EY. Molecular analysis of receptor protein tyrosine phosphatase mu-mediated cell adhesion. EMBO J. 2006 Feb 22;25(4):701-12. Epub 2006 Feb 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-KLIRQVDKWQEEYN
NCBI Gene ID #:
5797
NCBI Official Name:
protein tyrosine phosphatase, receptor type, M
NCBI Official Symbol:
PTPRM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001098714.1, NP_002836.3
Protein GI Number:
157419152
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001098714.1 and NP_002836.3).
Swissprot #:
P28827