PUM2 Antibody

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ProSci
Product Code: PSI-46-254
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-254-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed with 46-254 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-254 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed with 46-254 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-254 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
PUM2, PUMH2, PUML2, KIAA0235, pumilio homolog 2 (Drosophila), pumilio (Drosphila) homolog 2, FLJ36528, MGC138251, MGC138253, OTTHUMP00000200539, pumilio homolog 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration 0.3-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
Background References:
  • Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26;15(2):171-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DQNRDLKQGDDDDSK
NCBI Gene ID #:
23369
NCBI Official Name:
pumilio RNA-binding family member 2
NCBI Official Symbol:
PUM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056132.1
Protein GI Number:
13491168
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q8TB72