RAE1 Antibody

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ProSci
Product Code: PSI-46-264
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-264-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-264 (0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-264 (0.1ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
RAE1, RAE1 RNA export 1 homolog (S. pombe) , HGNC:9828, FLJ30608, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1, RAE1 (RNA export 1, S.pombe) homolog, homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer), mRNA export protein, mRNA-associated protein MRNP 41, mRNA-binding protein, 41-kD, migration-inducing gene 14, RP4-800J21.2, MGC117333, MGC126076, MGC126077, OTTHUMP00000166110
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
Background References:
  • Blower MD, Nachury M, Heald R, Weis K. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell. 2005 Apr 22;121(2):223-34.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NAAEELKPRNKK
NCBI Gene ID #:
8480
NCBI Official Name:
ribonucleic acid export 1
NCBI Official Symbol:
RAE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003601.1, NP_001015885.1
Protein GI Number:
4506399
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
NP_003601and NP_001015885 represent identical proteins.
Swissprot #:
P78406