RARA Antibody

ProSci
Product Code: PSI-46-267
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-267-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-267 (1ug/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-267 (1ug/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
RARA, RAR, NR1B1, retinoic acid receptor, alpha, Retinoic acid receptor, alpha polypeptide, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 50kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Background References:
  • Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R. Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity. J Natl Cancer Inst. 2005 Jan 5;97(1):21-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SPSLSPSSHRSSPATQSP
NCBI Gene ID #:
5914
NCBI Official Name:
retinoic acid receptor, alpha
NCBI Official Symbol:
RARA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000955.1, NP_001138773.1, NP_001019980.1, NP_001138774.1
Protein GI Number:
4506419
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.
Swissprot #:
P10276