RNF125 Antibody

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ProSci
Product Code: PSI-46-303
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-303-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-303 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum/Golgi and some nuclear stain
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46-303 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum/Golgi and some nuclear stain

Documents

Further Information

Additional Names:
RNF125, FLJ20456, MGC21737, ring finger protein 125, TRAC1, T-cell ring protein identified in activation screen
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum/Golgi of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Chu P, Pardo J, Zhao H, Li CC, Pali E, Shen MM, Qu K, Yu SX, Huang BC, Yu P, Masuda ES, Molineaux SM, Kolbinger F, Aversa G, De Vries J, Payan DG, Liao XC. Systematic identification of regulatory proteins critical for T-cell activation. J Biol. 2003;2(3):21. Epub 2003 Sep 15.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RSLLEYVNHSNTT
NCBI Gene ID #:
54941
NCBI Official Name:
ring finger protein 125, E3 ubiquitin protein ligase
NCBI Official Symbol:
RNF125
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060301.2
Protein GI Number:
37595555
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q96EQ8