RNF8 Antibody

ProSci
Product Code: PSI-46-313
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-313-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
46-313 (1ug/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 2

46-313 (1ug/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56kDa according to NP_003949.1 and 50.8kDa according to NP_898901.1 ). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T. Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif. J Hum Genet. 1998;43(4):272-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: GEPGFFVTGDRAG-C
NCBI Gene ID #:
9025
NCBI Official Name:
ring finger protein 8, E3 ubiquitin protein ligase
NCBI Official Symbol:
RNF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003949.1, NP_898901.1
Protein GI Number:
4504867
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
Swissprot #:
O76064