SIPA1 Antibody
| Code | Size | Price |
|---|
| PSI-46-371-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1, signal-induced proliferation-associated 1, MGC102688, signal-induced proliferation-associated gene 1, signal-induced proliferation-associated protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3ug/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
Background References:
- Hattori M, Tsukamoto N, Nur-e-Kamal MS, Rubinfeld B, Iwai K, Kubota H, Maruta H, Minato N. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol Cell Biol. 1995 Jan;15(1):552-60.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ASKQLGSPTADLA
NCBI Gene ID #:
6494
NCBI Official Name:
signal-induced proliferation-associated 1
NCBI Official Symbol:
SIPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_694985.28, NP_006738.3
Protein GI Number:
24497629
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Both variants represent identical protein (NP_694985.28 and NP_006738.3).
Swissprot #:
Q96FS4