SLC47A1 Antibody

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ProSci
Product Code: PSI-42-080
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-080-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-080 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-080 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
SLC47A1, solute carrier family 47, member 1, FLJ10847, MATE1, MGC64822, multidrug and toxin extrusion 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 60kDa band observed in Human Kidney lysates (calculated MW of 61.9kDa according to NP_060712.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Chen Y, Zhang S, Sorani M, Giacomini KM. Transport of paraquat by human organic cation transporters and multidrug and toxic compound extrusion family. J. Pharmacol. Exp. Ther. 2007 Aug 322 (2): 695-700.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PEHPQDGAKLSRKQ
NCBI Gene ID #:
55244
NCBI Official Name:
solute carrier family 47 (multidrug and toxin extrusion), member 1
NCBI Official Symbol:
SLC47A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060712.2
Protein GI Number:
22907060
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q96FL8