SNX5 Antibody

ProSci
Product Code: PSI-46-404
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-404-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-404 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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46-404 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Further Information

Additional Names:
SNX5, FLJ10931, sorting nexin 5, RP11-504H3.2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 48kDa band observed in Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3ug/ml. Primary incubation was one hour.Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration: 3-6ug/ml.
Background References:
  • Otsuki T, Kajigaya S, Ozawa K, Liu JM. SNX5, a new member of the sorting nexin family, binds to the Fanconi anemia complementation group A protein. Biochem Biophys Res Commun. 1999 Nov 30;265(3):630-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: SLLQSCIDLFKNN
NCBI Gene ID #:
27131
NCBI Official Name:
sorting nexin 5
NCBI Official Symbol:
SNX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055241.1, NP_001269383.1
Protein GI Number:
7657599
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_055241.1; NP_001269383.1). Reported variants represent identical protein: NP_055241.1, NP_689413.1
Swissprot #:
Q9Y5X3