SPI1 Antibody
| Code | Size | Price |
|---|
| PSI-42-746-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-DLYQRQTHEYY
NCBI Gene ID #:
6688
NCBI Official Name:
spleen focus forming virus (SFFV) proviral integration oncogene
NCBI Official Symbol:
SPI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001074016.1, NP_003111.2
Protein GI Number:
124028521
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
Swissprot #:
P17947