SRF Antibody

Contact us
ProSci
Product Code: PSI-42-777
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-777-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
42-777 (1ug/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin
❮❯

42-777 (1ug/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin

Documents

Further Information

Additional Names:
SRF, serum response factor (c-fos serum response element-binding transcription factor), MCM1, OTTHUMP00000039820
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590). Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QASPSRDSSTDLTQ
NCBI Gene ID #:
6722
NCBI Official Name:
serum response factor (c-fos serum response element-binding transcription factor)
NCBI Official Symbol:
SRF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003122.1
Protein GI Number:
4507205
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
P11831