STAP-2 Antibody

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ProSci
Product Code: PSI-46-439
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-439-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-439 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-439 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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46-439 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-439 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2, signal transducing adaptor family member 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Heart lysates and 45kDa band observed in Human Breast Lysates (calculated MW of 49.6kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5ug/ml.
Background References:
  • Minoguchi M, Minoguchi S, Aki D, Joo A, Yamamoto T, Yumioka T, Matsuda T, Yoshimura A. STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif. J Biol Chem. 2003 Mar 28;278(13):11182-9. Epub 2003 Jan 22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ELQKKLEKRRALEH
NCBI Gene ID #:
55620
NCBI Official Name:
signal transducing adaptor family member 2
NCBI Official Symbol:
STAP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060190.2, NP_001013863.1
Protein GI Number:
62243734
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
Swissprot #:
Q9UGK3