STS2 Antibody
| Code | Size | Price |
|---|
| PSI-46-446-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TULA , STS2 CBL-interacting protein 4, UBASH3A, ubiquitin associated and SH3 domain containing, A , T-cell ubiquitin ligand protein, Suppressor of T-cell receptor signaling 2, CLIP4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration: 2.5ug/ml.
Background References:
- Kowanetz K, Crosetto N, Haglund K, Schmidt MH, Heldin CH, Dikic I. Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases. J Biol Chem. 2004 Jul 30;279(31):32786-95. Epub 2004 May 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: QQCSTPDGKYYRPD
NCBI Gene ID #:
53347
NCBI Official Name:
ubiquitin associated and SH3 domain containing A
NCBI Official Symbol:
UBASH3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_061834.1, NP_001001895.1
Protein GI Number:
9507221
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).
Swissprot #:
P57075