TAGLN Antibody
| Code | Size | Price |
|---|
| PSI-46-467-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rabbit
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TAGLN, SM22, SMCC, WS3-10, transgelin, SM22-alpha, DKFZp686P11128, TAGLN1, DKFZp686B01212, transgelin variant 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 23-24kDa band observed in Human Testis lysates (calculated MW of 22.6kDa according to NP_003177.2 and NP_001001522.1). Preliminary testing also showed a band at approx 23kDa in lysates of cell line HeLa and approx 25kDa in Human Breast and Placenta lysates Recommended concentration: 0.1-1 ?g/ml. Primary incubation 1 hour at room temperature.
Background References:
- Nishida W, Kitami Y, Abe M, Hiwada K. Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle. Biochem Int. 1991 Mar;23(4):663-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-MTGYGRPRQIIS
NCBI Gene ID #:
6876
NCBI Official Name:
transgelin
NCBI Official Symbol:
TAGLN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003177.2
Protein GI Number:
48255905
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
NP_003177.2 and NP_001001522.1 represent identical protein.
The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.
Swissprot #:
Q01995