TFPI Antibody

ProSci
Product Code: PSI-45-160
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-160-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-160 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
2 / 2

45-160 staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Further Information

Additional Names:
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), TFPI, EPI, LACI, TFI, TFPI1, OTTHUMP00000163472, OTTHUMP00000205431, anti-convertin, extrinsic pathway inhibitor, lipoprotein-associated coagulation inhibitor, tissue factor pathway inhibitor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kDa to 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Wun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze GJ Jr. Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988 May 5;263(13):6001-4
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-VKIAYEEIFVKNM
NCBI Gene ID #:
7035
NCBI Official Name:
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
NCBI Official Symbol:
TFPI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006278.1
Protein GI Number:
5454114
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize isoform a (NP_006278.1) only.
Swissprot #:
P10646