TRIM71 Antibody

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ProSci
Product Code: PSI-42-541
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-541-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-541 (0.5ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-541 (0.5ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
TRIM71, tripartite motif-containing 71, LIN-41, LIN41, abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and of 92.1kDa according to Mouse NP_001035968.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 14kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NKLESTISAVQ
NCBI Gene ID #:
131405
NCBI Official Name:
tripartite motif containing 71, E3 ubiquitin protein ligase
NCBI Official Symbol:
TRIM71
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001034200.1
Protein GI Number:
84993742
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q2Q1W2