UCHL1 Antibody

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ProSci
Product Code: PSI-42-816
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-816-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-816 (0.01ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-816 (0.01ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
UCHL1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), PARK5, PGP9.5, PGP95, Uch-L1, neuron cytoplasmic protein 9.5, ubiquitin C-terminal hydrolase, ubiquitin carboxyl-terminal esterase L1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum and Hippocampus) and Mouse and Rat Brain lysates (calculated MW of 24.8kDa according to Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB10220.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QHENFRKKQIE
NCBI Gene ID #:
7345
NCBI Official Name:
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol:
UCHL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004172.2
Protein GI Number:
21361091
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P09936