VAV2 Antibody

Contact us
ProSci
Product Code: PSI-45-168
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-168-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
45-168 staining (1.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
2 / 2
❮❯

45-168 staining (1.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Documents

Further Information

Additional Names:
VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Henske EP, Short MP, Jozwiak S, Bovey CM, Ramlakhan S, Haines JL, Kwiatkowski DJ. Identification of VAV2 on 9q34 and its exclusion as the tuberous sclerosis gene TSC1. Ann Hum Genet. 1995 Jan;59 ( Pt 1):25-37.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: ETEAKYYRTLEDIEC
NCBI Gene ID #:
7410
NCBI Official Name:
vav 2 guanine nucleotide exchange factor
NCBI Official Symbol:
VAV2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001127870.1, NP_003362.2
Protein GI Number:
40549448
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).
Swissprot #:
P52735