XRCC5 Antibody

ProSci
Product Code: PSI-45-173
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-173-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-173 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-173 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
XRCC5, KU80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa) , HGNC:12833, KARP-1, KARP1, Ku86, NFIV , ATP-dependent DNA helicase II, DNA repair protein XRCC5, Ku86 autoantigen related protein 1, Ku autoantigen, 80kD, X-ray repair, complementing defective, repair in Chinese hamster
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of 75kDa was observed consistent with products from other sources.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Background References:
  • Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005 Mar 18;280(11):10827-33. Epub 2005 Jan 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SLAKKDEKTDTLED
NCBI Gene ID #:
7520
NCBI Official Name:
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
NCBI Official Symbol:
XRCC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_066964.1
Protein GI Number:
10863945
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
P13010