AADACL1 Antibody

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ProSci
Product Code: PSI-46-619
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-619-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-619 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Endoplasmic reticulum staining. The nuclear stain
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46-619 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Endoplasmic reticulum staining. The nuclear stain

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Further Information

Additional Names:
AADACL1, arylacetamide deacetylase-like 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 26-28kDa band in A431 cell lysates and in Human Hippocampus lysates after 1ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for the band, given the calculated size of 31.2kDa according to NP_001139749.1Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Background References:
  • Chiang KP, Niessen S, Saghatelian A, Cravatt BF. An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling. Chem Biol. 2006 Oct;13(10):1041-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RTRNSYIKWLDQN
NCBI Gene ID #:
57552
NCBI Official Name:
neutral cholesterol ester hydrolase 1
NCBI Official Symbol:
AADACL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065843.3, NP_001139748.2, NP_001139749.1
Protein GI Number:
68051721
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q6PIU2