APEX1 Antibody
| Code | Size | Price |
|---|
| PSI-27-662-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store APEX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Application Note:
APEX1 antibody can be used for detection of APEX1 by ELISA at 1:312500. APEX1 antibody can be used for detection of APEX1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.
Background References:
- Ding, S.Z., et al., (2004) Gastroenterology 127 (3), 845-858.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APEX1.
NCBI Gene ID #:
328
NCBI Official Name:
APEX nuclease (multifunctional DNA repair enzyme) 1
NCBI Official Symbol:
APEX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
NP_542379
Protein GI Number:
18375503
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P27695
User NOte:
Optimal dilutions for each application to be determined by the researcher.