CHST14 Antibody

ProSci
Product Code: PSI-30-488
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-30-488-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHST14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

Further Information

Additional Names:
CHST14, D4ST-1, D4ST1, HD4ST, HNK1ST, ATCS, EDSMC1
Application Note:
CHST14 antibody can be used for detection of CHST14 by ELISA at 1:312500. CHST14 antibody can be used for detection of CHST14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
CHST14 belongs to the sulfotransferase 2 family. CHST14 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. It transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. It transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. CHST14 has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHST14.
NCBI Gene ID #:
113189
NCBI Official Name:
carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
NCBI Official Symbol:
CHST14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
AAH23653
Protein GI Number:
40289703
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q8NCH0
User NOte:
Optimal dilutions for each application to be determined by the researcher.