EGLN1 Antibody

ProSci
Product Code: PSI-28-141
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-141-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EGLN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 1.25 ug/ml.

Antibody used in WB on Human HepG2 at 1.25 ug/ml.

Further Information

Additional Names:
EGLN1, HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2
Application Note:
EGLN1 antibody can be used for detection of EGLN1 by ELISA at 1:12500. EGLN1 antibody can be used for detection of EGLN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EGLN1 has a role in the regulation of hypoxia-inducible factor. It is not affected by overexpression or downregulation of HIF-2alpha.
Background References:
  • Appelhoff, R.J., et al., (2004) J. Biol. Chem. 279 (37), 38458-38465.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN1.
NCBI Gene ID #:
54583
NCBI Official Name:
egl-9 family hypoxia-inducible factor 1
NCBI Official Symbol:
EGLN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
NP_071334
Protein GI Number:
13489073
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
Q9GZT9
User NOte:
Optimal dilutions for each application to be determined by the researcher.