ENO1 Antibody

ProSci
Product Code: PSI-28-773
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-773-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • C. Elegans
  • Fish
  • Human
  • Plant
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ENO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

Further Information

Additional Names:
ENO1, NNE, PPH, MPB1, ENO1L1
Application Note:
ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Background References:
  • Ghosh, A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO1.
NCBI Gene ID #:
2023
NCBI Official Name:
enolase 1, (alpha)
NCBI Official Symbol:
ENO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
NP_001419
Protein GI Number:
4503571
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P06733
User NOte:
Optimal dilutions for each application to be determined by the researcher.