TCF3 Antibody

ProSci
Product Code: PSI-28-832
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-832-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 cells at 1 ug/ml.

Antibody used in WB on Human HepG2 cells at 1 ug/ml.

Further Information

Additional Names:
TCF3, E2A, E47, ITF1, VDIR, TCF-3, bHLHb21
Application Note:
TCF3 antibody can be used for detection of TCF3 by ELISA at 1:1562500. TCF3 antibody can be used for detection of TCF3 by western blot at 0.31 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Background References:
  • Maruyama, K. (2005) J. Biol. Chem. 280 (41), 34577-34589.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF3.
NCBI Gene ID #:
6929
NCBI Official Name:
transcription factor 3
NCBI Official Symbol:
TCF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
NP_003191
Protein GI Number:
27777636
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P15923
User NOte:
Optimal dilutions for each application to be determined by the researcher.