ASRGL1 Antibody

ProSci
Product Code: PSI-46-628
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-628-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-628 (0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-628 (0.5ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ASRGL1, ALP, asparaginase like 1, ALP1, FLJ22316, asparaginase-like 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Bush LA, Herr JC, Wolkowicz M, Sherman NE, Shore A, Flickinger CJ. A novel asparaginase-like protein is a sperm autoantigen in rats. Mol Reprod Dev. 2002 Jun;62(2):233-47.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: EKHEKGAQKTDCQ
NCBI Gene ID #:
80150
NCBI Official Name:
asparaginase like 1
NCBI Official Symbol:
ASRGL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001077395.1, NP_079356.3
Protein GI Number:
145275202
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Both reported variants represent identical protein (NP_001077395.1 and NP_079356.3).
Swissprot #:
Q7L266