CES1 Antibody

ProSci
Product Code: PSI-46-640
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-640-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-640 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-640 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn, liver carboxylesterase, triacylglycerol hydrolase, monocyte/macrophage serine-esterase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.
Background References:
  • Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E958-64. Epub 2007 Jul 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-NTQAAQKLKDKE
NCBI Gene ID #:
1066
NCBI Official Name:
carboxylesterase 1
NCBI Official Symbol:
CES1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001020366.1, NP_001020365.1, NP_001257.4
Protein GI Number:
68508967
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
Swissprot #:
P23141