ProSci

DTX2 Antibody

Product Code:
 
PSI-42-781
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
42-781 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-781 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-42-781-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DTX2, deltex homolog 2 (Drosophila), KIAA1528, MGC71098, RNF58, OTTHUMP00000024490, deltex 2, ring finger protein 58, zinc ion binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 85kDa band in lysates of cell lines A549, HepG2 and NIH3T3 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.2kDa according to NP_065943.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-EKLSTASGYSDVTD
NCBI Gene ID #:
113878
NCBI Official Name:
deltex 2, E3 ubiquitin ligase
NCBI Official Symbol:
DTX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065943.2, NP_001096066.1
Protein GI Number:
156564380
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_065943.2; NP_001096066.1). Reported variants NP_001096065.1, NP_065943.2, NP_001096064.1 represent identical protein.
Swissprot #:
Q86UW9