GAD67 Antibody

ProSci
Product Code: PSI-46-779
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-779-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-779 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-779 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
GAD1, glutamate decarboxylase 1 (brain, 67kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
Background References:
  • Huang HS, Akbarian S. GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia. PLoS ONE 2007 2 (8): e809.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-PDSPQRREKLHK
NCBI Gene ID #:
2571
NCBI Official Name:
glutamate decarboxylase 1 (brain, 67kDa)
NCBI Official Symbol:
GAD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000808.2
Protein GI Number:
58331246
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Immunology
SPECIFICITY:
This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Swissprot #:
Q99259