IGFBP3 Antibody

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ProSci
Product Code: PSI-46-676
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-676-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-676 (0.02ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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46-676 (0.02ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
IGFBP3, insulin-like growth factor binding protein 3, tcag7.703, BP-53, IBP3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex , binding protein 29, binding protein 53, growth hormone-dependent binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in Human Breast Cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06ug/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Background References:
  • Lin J, Manson JE, Lee IM, Cook NR, Buring JE, Zhang SM. Intakes of calcium and vitamin D and breast cancer risk in women. Arch Intern Med. 2007 May 28;167(10):1050-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RYKVDYESQSTDTQN
NCBI Gene ID #:
3486
NCBI Official Name:
insulin-like growth factor binding protein 3
NCBI Official Symbol:
IGFBP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001013416.1, NP_000589.2
Protein GI Number:
62243248
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).
Swissprot #:
P17936