LIG1 Antibody

ProSci
Product Code: PSI-42-770
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-770-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-770 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-770 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-RVREDKQPEQATTS
NCBI Gene ID #:
3978
NCBI Official Name:
ligase I, DNA, ATP-dependent
NCBI Official Symbol:
LIG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000225.1
Protein GI Number:
4557719
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P18858